Invention of Propoxur Identification Method as a Cause of Human Death Using Necrophage Insects
Invention of Propoxur Identification Method as a Cause of Human Death Using Necrophage Insects |
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| © 2025 by IJETT Journal | ||
| Volume-73 Issue-4 |
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| Year of Publication : 2025 | ||
| Author : Sri Adelila Sari, Yeni Rossito Hutasuhut, Lia Mardiana Nasution, Hanisah Hasibuan, Ho Soon Min |
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| DOI : 10.14445/22315381/IJETT-V73I4P110 | ||
How to Cite?
Sri Adelila Sari, Yeni Rossito Hutasuhut, Lia Mardiana Nasution, Hanisah Hasibuan, Ho Soon Min, "Invention of Propoxur Identification Method as a Cause of Human Death Using Necrophage Insects," International Journal of Engineering Trends and Technology, vol. 73, no. 4, pp. .104-113, 2025. Crossref, https://doi.org/10.14445/22315381/IJETT-V73I4P110
Abstract
Cases of unnatural death, when the cause was unknown, were often followed by an autopsy to determine the cause of death. However, sometimes the victim's family refused the autopsy for various reasons, leading to many cases remaining unresolved. Deaths caused by illegal drugs, such as propoxur, could be analyzed using forensic entomology, which used necrophagous insect larvae to analyze the time of death when the body's internal organs had been degraded, and no usable blood or urine remained. The study aimed to analyze the levels of propoxur [2-isopropoxyphenyl N-methyl carbamate] as a cause of death by using necrophage insects. Maggot samples were extracted and analyzed using GC-MS, including those cultivated on fresh beef as a blank and meat mixed with propoxur as the test sample. The linearity, LOD, and LOQ were ascertained during the procedure. The concentration of propoxur in the sample was found to be 62.759 ppm, with a peak area of 259,904 and a retention time of 4.107 minutes. The linear regression equation y = 10906x – 424556 was used to determine linearity, and the R2 value was 0.9913. Furthermore, it was discovered that the LOD and LOQ values were, respectively, 13.48 mg/L and 44.96 mg/L. The study concluded that, in forensic applications, the simpler and more effective autopsy approach might be replaced by the forensic entomo-toxicology method employing maggots.
Keywords
Forensic entomology, GCMS, Manggot, Medicine, and Propoxur.
References
[1] Naji Arafat Mahat, Mohamed Afiq, and Mohamed Huri, Scientific Approaches to Crime Investigations, Penerbit UTM Press, pp. 37-89, 2019.
[Google Scholar] [Publisher Link]
[2] Jason H. Byrd, and James L. Castner, Insects of Forensic Importance, Forensic Entomology, CRC Press, Boca Raton, Florida, USA, pp. 35-60, 2001.
[Google Scholar] [Publisher Link]
[3] Jiri Hodecek, “Revisiting the Concept of Entomotoxicology,” Forensic Science International: Synergy, vol. 2, pp. 282-286, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[4] Terry L. Eberhardt, and Douglas A. Elliot, “A Preliminary Investigation of Insect Colonisation and Succession on Remains in New Zealand,” Forensic Science International, vol. 176, no. 2-3, pp. 217-223, 2008.
[CrossRef] [Google Scholar] [Publisher Link]
[5] David B. Rivers, and Gregory A. Dahlem, The Science of Forensic Entomology, Wiley, Hoboken, New Jersey, United States, pp. 37-74, 2014.
[CrossRef] [Google Scholar] [Publisher Link]
[6] N.A. Mahat, P.T. Jayaprakash, and Z. Zafarina, “Malathion Extraction from Larvae of Chrysomya Megacephala (Fabricius) (Diptera: Calliphoridae) for Determining Death Due to Malathion,” Tropical Biomedicine, vol. 29, no. 1, pp. 9-17, 2012.
[Google Scholar] [Publisher Link]
[7] Dorothy Gennard, Forensic Entomology: An Introduction, 2nd Ed., Wiley-Blackwell, 2012.
[Google Scholar] [Publisher Link]
[8] Haribhau M. Pawar, and Gajanan M. Deshmukh, “Effect of Alprazolam on the Developmental Stages of Forensically Important blow Fly Chrysomya Megacephala (Diptera: Calliphoridae),” Journal of Entomology and Zoology Studies, vol. 10, no. 3, pp. 101-104, 2022.
[CrossRef] [Google Scholar] [Publisher Link]
[9] Abdul Gafar Parinduri, Textbook of Forensic & Medicolegal Medicine: A Guide for Medical Students, Medan, UMSU PRESS 2020.
[Google Scholar] [Publisher Link]
[10] I. Dicky Faizal, B. Aswin Djoko, and Bambang Sidharta, “Exposure to Lethal Doses of Morphine in Rat Carcasses on the Growth of Sarcophaga Sp Larvae,” Brawijaya Medical Journal, vol. 26, no. 4, pp. 227-232, 2011.
[CrossRef] [Google Scholar] [Publisher Link]
[11] Hadi Alamri, “Position of Expert Statement as Evidence According to the Criminal Procedure Code,” Lex Privatum, vol. 5, no. 1, pp. 31-38, 2017.
[Google Scholar] [Publisher Link]
[12] Agustin Citra Dwi Handoko, “Determining Post Mortem Interval (PMI) by Examination of Insect Larvae Growth Based on Death Causative Factors,” Indonesian Scientific Journal, vol. 6, no. 2, pp. 1044-1054, 2021.
[CrossRef] [Google Scholar] [Publisher Link]
[13] Abdul Mun'im Idries, and Agung Legowo Tjiptomartono, “The Role of Forensic Medicine in the Investigation Process,” Sagung Seto, 2008.
[Google Scholar]
[14] Erwin Kristanto, Sunny Wangko, Sonny Kalangi, and Johannis Mallo, “The Role of Forensic Entomology in Estimating the Time of Death and Medical Crime Scene Investigation (Introduction to Medical Entomology),” Biomedical Journal: JBM, vol. 1, no. 1, pp. 1-4, 2009.
[CrossRef] [Google Scholar] [Publishker Link]
[15] E.C. Pastula, and R.W. Merritt, “Insect Arrival Pattern and Succession on Buried Carrion in Michigan,” Journal of Medical Entomology, vol. 50, no. 2, pp. 432-439, 2013.
[CrossRef] [Google Scholar] [Publisher Link]
[16] Michelle L. Harvey, Natalie E. Gasz, and Sasha C. Voss, “Entomology-Based Methods for Estimation of Postmortem Interval,” Research and Reports in Forensic Medical Science, vol. 6, pp. 1-9, 2016.
[CrossRef] [Google Scholar] [Publisher Link]
[17] F. Introna et al., “Time Since Death Definition by Experimental Reproduction of Lucilia Sericata Cycles in Growth Cabinet,” Journal of Forensic Sciences, vol. 34, no. 2, pp. 478-480, 1989.
[CrossRef] [Google Scholar] [Publisher Link]
[18] H.G. Gowtham et al., “Harnessing Abiotic Elicitors to Bolster Plant’s Resistance against Bacterial Pathogens,” Plant Stress, vol. 11, pp. 1-15, 2024.
[CrossRef] [Google Scholar] [Publisher Link]
[19] Patrick Morris, and T. Maniam, “Ethnicity and Suicidal Behaviour in Malaysia: A Review of the Literature,” Transcultural Psychiatry, vol. 38, no. 1, pp. 51-63, 2001.
[CrossRef] [Google Scholar] [Publisher Link]
[20] David Gunnell, and Michael Eddleston, “Suicide by Intentional Ingestion of Pesticides: A Continuing Tragedy in Developing Countries,” International Journal of Epidemiology, vol. 32, no. 6, pp. 902-909, 2003.
[CrossRef] [Google Scholar] [Publisher Link]
[21] M. Islambulchilar, Z. Islambulchilar, and MH Kargar-Maher, “Acute Adult Poisoning Cases Admitted to a University Hospital in Tabriz, Iran,” Human and Experimental Toxicology, vol. 28, no. 4, pp. 185-190, 2009.
[CrossRef] [Google Scholar] [Publisher Link]
[22] T. Maniam, “Suicide and Parasuicide in a Hill Resort in Malaysia,” The British Journal of Psychiatry, vol. 153, no. 2, pp. 222-225, 1988.
[CrossRef] [Google Scholar] [Publisher Link]
[23] A.M. Tsatsakis et al., “Pesticide Desposition in Hair: Preliminary Results of a Model Study of Emthomyl Incorporation into Rabbit Hair,” Veterinary and Human Toxicology, vol. 40, no. 4, pp. 200-203, 1998.
[Google Scholar] [Publisher Link]
[24] Azmi Wijayati, Ahmad Basori, and Mochammad Yuwono, “Propoxur Analysis in Postmortem Blood Samples of Wistar Rats,” Journal of Postgraduate Biosciences, vol. 19, no. 1, pp. 27-40, 2017.
[CrossRef] [Google Scholar] [Publisher Link]
[25] Matthew J. Ellenhorn, and Donald G. Barceloux, Medical Toxicology: Diagnosis and Treatment of Human Poisoning, Elsevier, pp. 1-512, 1988.
[Google Scholar] [Publisher Link]
[26] Stephen Brimijoin, “Enzymology and Biology of Cholinesterases,” U.S. EPA Workshop on Cholinesterase Methodologies, Abstracts, Sheraton Crystal City, Arlington, Virginia, United States Environmental Protection Agency, 1991.
[Publisher Link]
[27] Milan Jokanovic, “Medical Treatment of Acute Poisoning with Organophosphorus and Carbamate Pesticides,” Toxicology Letters, vol. 190, no. 2, pp. 107-115, 2009.
[CrossRef] [Google Scholar] [Publisher Link]
[28] Enviromental Protecting Agency, Propoxur: Risk Characterization Document, California, EPA, 1992. [Online]. Available: https://archive.epa.gov/pesticides/reregistration/web/pdf/2555red.pdf [29] Lucio G. Costa et al., “Tolerance to the Carbamate Insecticide Propoxur,” Toxicology, vol. 21, no. 4, pp. 267-278, 1981.
[CrossRef] [Google Scholar] [Publisher Link]
[30] Faizal Arief Nurokhman, Ahmad Basori, and M. Yuwono, “Analysis of Propoxur LD50 Analysis on the Growth of Sarcophaga sp. Fly Larvae Using Gas Chromatography-Mass Spectrometry,” Journal of Postgraduate Biosciences, vol. 20, no. 2, pp. 93-106, 2018.
[CrossRef] [Google Scholar] [Publisher Link]
[31] Ruchi Sharma, Rakesh Kumar Garg, and J.R. Gaur, “Various Methods for the Estimation of the Post Mortem Interval from Calliphoridae: A Review,” Egyptian Journal of Forensic Sciences, vol. 5, no. 1, pp. 1-12, 2015.
[CrossRef] [Google Scholar] [Publisher Link]
[32] Jason H. Byrd, and Jeffery K. Tomberlin, Forensic Entomology: The Utility of Arthropods in Legal Investigations, CRC Press, New York, USA, 2019.
[Google Scholar] [Publisher Link]
[33] Jason H. Byrd, and James L. Castner, Forensic Entomology: The Utility of Arthropods in Legal Investigations, CRC Press, 2009.
[CrossRef] [Publisher Link]
[34] J. Amendt et al., “Forensic Entomology: Applications and Limitations,” Forensic Science, Medicine, and Pathology, vol. 7, pp. 379-392, 2011.
[CrossRef] [Google Scholar] [Publisher Link]
[35] Y. Wang et al., “Development of the Forensically Important Beetle Creophilus Maxillosus (Coleoptera: Staphylinidae) at Constant Temperatures,” Journal of Medical Entomology, vol. 54, no. 2, pp. 281-289, 2017.
[CrossRef] [Google Scholar] [Publisher Link]
[36] Hong Xu et al., “Age-Dependent Changes in Cuticular Hydrocarbons of Larvae in Aldrichina Grahami (Aldrich) (Diptera: Calliphoridae),” Forensic Science International, vol. 242, pp. 236-241, 2014.
[CrossRef] [Google Scholar] [Publisher Link]
[37] E.P. Catts, and M.L. Goff, “Forensic Entomology in Criminal Investigations,” Annual Review of Entomology, vol. 37, no. 1, pp. 253-272, 1992.
[CrossRef] [Google Scholar] [Publisher Link]
[38] Leonardo Gomes, Wesley Augusto Conde Godoy, and Claudio José Von Zuben, “A Review of Postfeeding Larval Dispersal in Blowflies: Implications for Forensic Entomology,” Naturwissenschaften, vol. 93, no. 5, pp. 207-215, 2006.
[CrossRef] [Google Scholar] [Publisher Link]
[39] Sundus Mona et al., “Forensic Entomology: A Comprehensive Review,” Advancements in Life Sciences, vol. 6, no. 2, pp. 48-59, 2019.
[CrossRef] [Google Scholar] [Publisher Link]
[40] Paola A. Magni et al., “Development and Validation of a GC-MS Method for Nicotine Detection in Calliphora Vomitoria (L) (Diptera: Calliphoridae),” Forensic Science International, vol. 261, pp. 53-60, 2016.
[CrossRef] [Google Scholar] [Publisher Link]
[41] Rito Chophi et al., “Forensic Entomotoxicology: Current Concepts, Trends and Challenges,” Journal of Forensic and Legal Medicine, vol. 67, pp. 28-36, 2019.
[CrossRef] [Google Scholar] [Publisher Link]
[42] J.C. Beyer, W.F. Enos, and M. Stajić, “Drug Identification through Analysis of Maggots,” Journal of Forensic Sciences, vol. 25, no. 2, pp. 411-412, 1980.
[CrossRef] [Google Scholar] [Publisher Link]
[43] Hermansyah Hermansyah, Kenedy Putra, and Lilis Riyanti, “Utilization of Pineapple Skin as a Growth Medium for Black Soldier Fly Maggots,” Triton Journal, vol. 14, no. 1, pp. 10-17, 2023.
[CrossRef] [Google Scholar] [Publisher Link]
[44] Tri Puji Rahayu, Esna Dilla Novianto, and Candarisma Dhanes Noor Viana, “The Effect of Fermentation Time of Bran from Pineapple Peel Waste on the Biomass of Hermetia illucens Larvae,” Journal of Animal Science, vol. 8, no. 2, pp. 114-121, 2020.
[Google Scholar] [Publisher Link]
[45] Tim Penulis PS, Waste Handling and Processing, Penebar Swadaya, 2008.
[Google Scholar] [Publisher Link]
[46] Kis Dewantoro DKK et al., Berternak Maggot Black Soldier Fly, Kawah Media Library Pt 1, pp. 1-96, 2018.
[Google Scholar] [Publisher Link]
[47] Rizkia Suciati, “The Effectiveness of the Growth Media of Maggot Hermetia Illucens (Black Soldier Fly) as a Solution for Utilizing Organic Waste,” Biosphere: Journal of Biology and Biology Education, vol. 2, no. 1, pp. 8-13, 2017.
[CrossRef] [Google Scholar] [Publisher Link]
[48] Lena Monita et al., “Urban Organic Waste Processing Using Black Soldier Fly Larvae (Hermetia Illucens),” Journal of Natural Resources and Environmental Management, vol. 7, no. 3, pp. 227-234, 2017.
[CrossRef] [Google Scholar] [Publisher Link]
[49] Nurcholis Salman, Estin Nofiyanti, and Tazkia Nurfadhilah, “The Influence and Effectiveness of Maggots as an Alternative Process for Decomposing Organic Waste in Municipalities in Indonesia,” Serambi Engineering Journal, vol. 5, no. 1, pp. 835-841, 2020.
[CrossRef] [Google Scholar] [Publisher Link]
[50] Frid Agustinus, and Infa Minggawati, “The Effect of Utilizing Banana Stems (Musa Paradisiaca) With Different Compositions to Grow Maggots (Hermetia Illucens),” Journal of Tropical Animal Science, vol. 8, no. 1, pp. 9-12, 2019.
[Google Scholar] [Publisher Link]
[51] D. Craig Sheppard et al., “A Value Added Manure Management System Using the Black Soldier Fly,” Bioresecrh Technology, vol. 50, no. 3, pp. 275-279, 1994.
[CrossRef] [Google Scholar] [Publisher Link]
[52] Arief Sabdo Yuwono, and Pricillia Dana Mentari, Use of Black Soldier Fly (BSF) Larvae in Organic Waste Processing, Seameo Biotrop, Bogor, 2018.
[Google Scholar] [Publisher Link]
[53] Lukman Junaidi, Natural Active Ingredients Extraction Technology, PT Penerbit IPB Press, 2020.
[Google Scholar]
[54] Anindi Lupita Nasyanka, Janatun Na'imah, and Riskha Aulia, Pengantar Fitokimia D3 Farmasi 2020, Penerbit Qiara Media, pp. 1-111, 2022.
[Google Scholar] [Publisher Link]
[55] Zidane Qriouet et al., “Analytical Methods Used for the Detection and Quantification of Benzodiazepines,” Journal of Analytical Methods in Chemistry, vol. 2019, pp. 1-11, 2019.
[CrossRef] [Google Scholar] [Publisher Link]
[56] Anita Sari, “Liquid-Liquid Extraction using EDTA Placer to Increase Zingibern Level in Ginger Essential Oil,” Diponegoro University, 2017.
[Google Scholar]
[57] Arisma Yanti et al., “Optimization of Ethanol and Methanol Content Determination Method in Mixed Alcoholic Beverages Using Gas Chromatography (GC),” Indonesian Journal of Chemical Science, vol. 8, no. 1, pp. 53-59, 2019.
[Google Scholar] [Publisher Link]
[58] Pratomo Setyadi Setyadi et al., “Design of Multi Speed Centrifuge as a Liquid Separation Tool,” Proceedings of the National Seminar on Community Service, pp. 250-260, 2021.
[Publisher Link]
[59] Abdullah Kunta Arsa, and Zubaidi Achmad, “Extraction of Essential Oil from Black Ginger Rhizome (Curcuma aeruginosa Roxb) with Ethanol and N-Hexane Solvents,” Technoscientia Technology Journal, vol. 13, no. 1, pp. 83-94, 2020.
[Google Scholar]
[60] H. Poedji Loekitowati, Fahma Fahma, and Asmaripa Aini, “The Effect of Type and Volume of Solvent on the Extraction Results of BHA and BHT from Cooking Oil,” Journal of Science Research, no. 14, 2003.
[Google Scholar] [Publisher Link]
[61] Oleg B. Rudakov, Liudmila V. Rudakova, and Vladimir F. Selemenev, “Acetonitrile as tops Solvent for Liquid Chromatography and Extraction,” Journal of Analytical Chromatography and Spectroscopy, vol. 1, no. 10, pp. 1-19. 2022.
[CrossRef] [Google Scholar] [Publisher Link]
[62] Dwi Hastuti, Rohadi Rohadi, and Dan Aldila Sagitaning Putri, “The Ratio of n-Hexane-Ethanol to the Physical and Chemical Characteristics of Ginger Dregs (Zingiber majus Rumph) Emprit Variety,” Journal of Food Technology and Research Results, vol. 13, no. 1, pp. 41-56, 2018.
[CrossRef] [Google Scholar] [Publisher Link]
[63] Rizki Amalia, and Mirwa Adiprahara Anggarani, “Analysis of Phenolic, Flavonoid Content and Antioxidant Activities of Onion Bulb (Allium cepa L.),” UNESA Journal of Chemistry, vol. 11, no. 1, pp. 34-45, 2022.
[CrossRef] [Publisher Link]
[64] Nabilla Riski Hanura, “Comparison of Extraction Methods in Determining THC (Tetrahydrocannabinol) Content in Dry Cannabis Leaf Samples (Cannabis Sativa L.) Using GC-MS,” Jambi University, 2022.
[Google Scholar] [Publisher Link]
[65] Ibnu Gholib Gandjar, and Abdul Rohman, “Kimia Farmasi Analisis,” Yogyakarta: Student Library, 2007.
[Google Scholar]
[66] Tania Danar Novianda, “Comparison of Preparation Methods for Determining Methamphetamine Content in Crystal Methamphetamine Samples Using Gas Chromatography MASS Spectrometry (GC-MS),” Undergraduate Thesis, Universitas Islam Indonesia, pp. 1-83, 2022.
[Google Scholar] [Publisher Link]
[67] Bambang Wispriyono, Arry Yanuar, and Laila Fitria, “Safety Level of Consumption of Carbamate Residues in Fruits and Vegetables According to Post-Column Analysis of High-Performance Liquid Chromatography,” Kesmas: National Public Health Journal, vol. 7, no. 7, pp. 317-323, 2013.
[CrossRef] [Google Scholar] [Publisher Link]
[68] Imas Solihat et al., “Verification of Testing Method for Nitrite Levels in Wastewater by UV-Visible Spectrophotometry,” Journal of Chemical Research, vol. 8, no. 1, pp. 53-59, 2022.
[Google Scholar]
[69] Ahmad Akhib Ainul Yaqin et al., “Determination of LoD and LoQ Values in Methanol Testing in Mixed Liquor Using Gas Chromatography with Various Methods,” Indonesian Journal of Chemical Science, vol. 8, no. 3, pp. 147-152, 2019.
[Google Scholar] [Publisher Link]
[70] Puji Purnama, and Dyah Ika Kusumaningtyas, “Determination of Detection Limit (Lod) and Quantitation Limit (Loq) in Phosphate (Po4-P) Measurement in Fresh Water Using the Ascorbic Acid Method,” Resource Engineering and Capture Research Bulletin, vol. 12, no. 1, pp. 71-75, 2016.
[Google Scholar]
[71] Harmita Harmita, “Instructions for Implementing Method Validation and Calculation Methods,” Pharmaceutical Science Magazine, vol. 1, no. 3, pp. 117-135, 2004.
[CrossRef] [Google Scholar] [Publisher Link]
[72] Zhihong Shi et al., “Graphene‐Based Pipette Tip Solid‐Phase Extraction with Ultra‐High Performance Liquid Chromatography and Tandem Mass Spectrometry for the Analysis of Carbamate Pesticide Residues in Fruit Juice,” Journal of Separation Science, vol. 39, no. 22, pp. 4391-4397, 2016.
[CrossRef] [Google Scholar] [Publisher Link]
[73] Y. Jabali, M. Millet, and M. El-Hoz, “Optimization of a DI-SPME-GC-MS/MS Method for Multi-Residue Analysis of Pesticides in Waters,” Microchemical Journal, vol. 147, pp. 83-92, 2019.
[CrossRef] [Google Scholar] [Publisher Link]