Approach and Techniques for Precise Prediction of N-Linked Glycosylation from Human Protein using Artificial Intelligence

Approach and Techniques for Precise Prediction of N-Linked Glycosylation from Human Protein using Artificial Intelligence

© 2022 by IJETT Journal
Volume-70 Issue-12
Year of Publication : 2022
Author : Mubina Malik, Jaimin N Undavia
DOI : 10.14445/22315381/IJETT-V70I12P213

How to Cite?

Mubina Malik, Jaimin N Undavia, "Approach and Techniques for Precise Prediction of N-Linked Glycosylation from Human Protein using Artificial Intelligence," International Journal of Engineering Trends and Technology, vol. 70, no. 12, pp. 118-126, 2022. Crossref,

Glycosylation is the most common post-translational modification of protein in all territories, which plays a significant role in biological processes. Amongst them, n-linked glycosylation is the most crucial modification, which is closely related to certain diseases such as cancer, diabetes, HIV infection, Alzheimer's disease and atherosclerosis, and liver cirrhosis. Recent advancements in biological knowledge are depicted in this article, ultimately targeting the computer science field. Machine learning and deep learning techniques are major keys to predicting various protein modifications. Through the review of several models which have been made existing for prediction and show high accuracy but result as false positives due to the poor biological knowledge, updated datasets and techniques used. Targeting precise prediction, drawbacks of the existing model and discussed parameters and techniques were emphasized to model solution in this paper. In this study, databases were combined, namely UniprotKB, dbPTM, and nGlycositeAtlas, which are experimentally verified and updated with window size 21. This window size is best for the n-linked glycosylation. After combining datasets and removing the redundancy, 11254 unique proteins and 33859 glycosites were received for further study. CD-HIT algorithm was implemented to remove the redundancy with threshold 0.9. These nearby locations for similar pattern sequences have been identified for asparagine residue for n-linked glycosylation. The protein sequence is a combination of 20 amino acids, which were required to convert into numerical form through encoding methods. Various encoding methods have conversed for n-linked glycosylation. With the biological features, amino acid encoding methods such as substitution matrices - Position Specific Scoring Matrix (PSSM) and Physicochemical properties encoding VHSE8 are the vital methods which improve the accuracy in n-linked glycosylation prediction.

Artificial intelligence, Deep learning, Human protein, Machine learning, N-linked glycosylation.

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