Comparative Analysis of Gene Prediction Tools: RAST, Genmark hmm and AMIgene
Chander Jyoti, Sandeep Saini, Varinder Kumar, Kajal Abrol, Kanchan Pandey, Ankit Sharma "Comparative Analysis of Gene Prediction Tools: RAST, Genmark hmm and AMIgene", International Journal of Engineering Trends and Technology (IJETT), V43(4),234-237 January 2017. ISSN:2231-5381. www.ijettjournal.org. published by seventh sense research group
High throughput genome sequencing made large amount of genome data available to research community. Accurate gene structure prediction and annotation is the fundamental step towards the understanding of genome function. A large number of gene prediction tool and pipeline have been developed over the past year. To understand whether the prediction tools and pipeline are providing same or different result for the same genome or not, we have compared manually the gene prediction result of RAST (Rapid Annotations using Subsystems Technology), AMIgene (Annotation of MIcrobial Genes) and Genmark hmm for organism Mycoplasm genitalium in reference to Genbank CDS (Coding Sequence) or gene. During comparative analysis we have seen the similarity as well as variation in prediction result of each tool. Variation in prediction results were also seen in total number of CDS predicted, gene coordinate and gene length. We have tried to find the reason behind the variation in prediction result and try to relate our analysis with nowadays high throughput data analysis. These types of analysis are useful to annotate a newly sequenced genome.
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Gene Prediction, CDS, Annotation, Mycoplasm genitalium.